Reversible depressive effect of TNFa on a model of isolated perfused rat heartīV Nguyen1, M Guillouet2, MA Giroux-Metges2, G Gueret3, M Ould-Ahmed1, JP Pennec2 Our data suggest autophagy plays a protective role in organ dysfunction in sepsis. Furthermore, inhibition of autophagy by chloroquine during the CLP procedure resulted in elevation of serum AST levels, and significantly increased mortality in mice.Ĭonclusion Autophagy was induced in several organs over the time course of the CLP sepsis model and then the process was gradually completed to degradation of the components. Autolysosome formation as evaluated by both fusion of GFP-LC3 dots with LAMP1 immunohistochemistry and electron microscopy was also increased after the procedure. Autophagosome formation became maximal at 6 hours and declined by 24 hours after CLP. The ratio was particularly increased in the liver, heart and spleen. Results Autophagy demonstrated by the ratio of LC3-II/LC3-I was induced over the time course up to 24 hours after CLP. To investigate a precise role of autophagy in CLP-induced sepsis, chloroquine, an autophagy inhibitor, was administered to the CLp-operated mice, and blood chemistry, pathology of the liver and survival were evaluated. We examined the kinetics of autophagosome and autol ysosome formation which may explain the status of autophagy by western blotting, immunohistochemistry, and electron microscopy. Methods Sepsis was induced by cecal ligation and puncture (CLP) in mice. We confirm whether the autophagic process is accelerated or sustained in an acute phase of sepsis and we also determine its physiological role. We hypothesized that autophagy is induced during the septic condition, and contributes to protect from tissue damage which subsequently leads to organ dysfunction. Introduction Autophagy is well known as one of the biogenic responses against various stresses, which possesses the beneficial roles for survival, but little is known about the dynamics and its significance during the septic condition. Research Center, Chiba University, Chiba, JapanĬritical Care 2013, 17(Suppl 2):P1 (doi: 10.1186/cc11939) 'Graduate School of Medicine, Chiba University, Chiba, Japan 2Biomedical W Takahashi1, H Hatano2, H Hirasawa1, S Oda1 Protective role of autophagy in mouse cecal ligation and puncture-induced sepsis model 33rd International Symposium on Intensive Care and Emergency Medicineīrussels, Belgium, 19-22 March 2013 Published: 19 March 2013
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. Archives
December 2022
Categories |